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KMID : 0545120230330060840
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 6 p.840 ~ p.847
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Korean Ginseng Berry Polysaccharide Enhances Immunomodulation Activities of Peritoneal Macrophages in Mice with Cyclophosphamide-Induced Immunosuppression
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Choi Jeong-Un
Nam Ju-Hyun
Weerawan Rod-in
Chaiwat Monmai
Jang A-Yeong
You Sang-Guan
Park Woo-Jung
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Abstract
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Korean ginseng (Panax ginseng C. A. Meyer), a member of the Araliaceae family, is known as a traditional medicinal plant to have a wide range of health properties. Polysaccharides constitute a major component of Korean ginseng, and its berries exhibit immune-modulating properties. The purpose of this study was to investigate the immune effects of crude polysaccharide (GBPC) extracted from Korean ginseng berry on peritoneal macrophages in mice with cyclophosphamide (CY)- induced immunosuppression. BALB/c mice were divided into eight groups: normal control, normal control + CY, levamisole + CY, ginseng + CY, and four concentrations of 50, 100, 250, and 500mg/kg BW/day of GBPC + CY. Mice were orally administered with samples for 10 days. Immunosuppression was established by treating mice with CY (80 mg/kg BW/day) through intraperitoneal injection on days 4 to 6. The immune function of peritoneal macrophages was then evaluated. Oral administration of 500mg/kg BW/day GBPC resulted in proliferation, NO production, and phagocytosis at 100%, 88%, and 91%, respectively, close to the levels of the normal group (100%) of peritoneal macrophages. In CY-treated mice, GBPC of 50?500 mg/kg BW/day also dose-dependently stimulated the proliferation, NO production, and phagocytosis at 56?100%, 47?88%, and 53?91%, respectively, with expression levels of immune-associated genes, such as iNOS, COX?2, IL?1β, IL?6, and TNF?α, of about 0.32 to 2.87-fold, compared to those in the CY group. GBPC could be a potential immunomodulatory material to control peritoneal macrophages under an immunosuppressive condition.
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KEYWORD
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Immune system, ginseng berry, polysaccharides, macrophages
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